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KMID : 0614020020170010033
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Journal of Pharmaceutical Sciences (C.N.U.)
2002 Volume.17 No. 1 p.33 ~ p.39
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Effect of Leuprolide Acetate, Lorelin on Rat Brain and Cytotoxicity of SNU-685 Cancer Cell Lines
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Seo Seong-Kon
Kim Won-Sik
Myung Pyung-Keun
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Abstract
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Leuprolide acetate is a potent luteinizing hormone-releasing hormone analogue [leuprorelin, (des-Glyl0-D-Leu6-Pro-NHEt9)-LHRH acetate]. Lorelin depot, which was composed of leuprolide acetate, was designed for one-month release injectable and biodegradable microsphere of multiple high doses. Here we examined the effect of microsphere lorelin depot, in comparison with Takeda microsphere (Lucrin Depot). Lorelin (leuprolide 3.75 ㎎/㎏ of body weight) was administered subcutaneous to male rats. The rats were sacrificed after 42 days and morphological changes of brain were observed by light microscopy. Leuprolide acetate has been shown to have an inhibitory effect on the growth of several hormone-dependent human tumors, So, leuprolide actate was examined for the cell cytotoxicity of the human endometrial cancer cell line, SNU-685, which was characterized in primary tumors of Korean patient and other cancer cell lines, MCF-7, T-24 and FB-l3P Cells were cultured with indicated concentrations of leuprolide acetate for 48 hr. Antiproliferative effect on SNU-685 cell which were determined by colorimetric method, MTT and MTS/PMS, was shown 20%, but those effects on MCF-7, T24, FB-23P cells were little shown.
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KEYWORD
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Leuprolide acetate, Lorelin, morphology, SNU-685 cell, cytotoxicity
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